Shenzhenlvshiyuan Biotechnology Co.,Ltd

1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in chicken, fish, shrimp, milk, honey, whole egg. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Tissue, honey, egg 0.2 ppb
Fish, shrimp (some interference) 0.3 ppb
Recovery rate
Shrimp and fish 85±10%
Honey ,chicken meat /liver,whole egg 75±15%
Cross-reaction rate
SEM 100%
AHD ?0.1%
AMOZ ?0.1%
AOZ ?0.1%

3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3) Enzyme conjugate (12 mL) red cap
4) Antibody working solution (7 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) .yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) 2× concentrated redissolving solution (50 mL) transparent cap
10) 2-Nitrobenzaldehyde(C7H5NO3) solution (10 mL) white

From: Shenzhenlvshiyuan Biotechnology Co.,Ltd
Posted on: 6/26/2011 5:18:19 PM
Expiring On: 12/23/2011 5:18:19 PM
Days Left: (-55 days to go!)